In planta proximity dependent biotin identification (BioID)
نویسندگان
چکیده
منابع مشابه
An improved smaller biotin ligase for BioID proximity labeling
The BioID method uses a promiscuous biotin ligase to detect protein-protein associations as well as proximate proteins in living cells. Here we report improvements to the BioID method centered on BioID2, a substantially smaller promiscuous biotin ligase. BioID2 enables more-selective targeting of fusion proteins, requires less biotin supplementation, and exhibits enhanced labeling of proximate ...
متن کاملIdentification of Interactions in the NMD Complex Using Proximity-Dependent Biotinylation (BioID)
Proximity-dependent trans-biotinylation by the Escherichia coli biotin ligase BirA mutant R118G (BirA*) allows stringent streptavidin affinity purification of proximal proteins. This so-called BioID method provides an alternative to the widely used co-immunoprecipitation (co-IP) to identify protein-protein interactions. Here, we used BioID, on its own and combined with co-IP, to identify protei...
متن کاملProbing mammalian centrosome structure using BioID proximity-dependent biotinylation.
Understanding the structure and function of the centrosome will require identification of its constituent components and a detailed characterization of the interactions among these components. Here, we describe the application of proximity-dependent biotin identification (BioID) to identify spatial and temporal relationships among centrosome proteins. The BioID method relies on protein fusions ...
متن کاملMeet the neighbors: Mapping local protein interactomes by proximity‐dependent labeling with BioID
Proximity-dependent biotin identification (BioID) is a recently developed method that allows the identification of proteins in the close vicinity of a protein of interest in living cells. BioID relies on fusion of the protein of interest with a mutant form of the biotin ligase enzyme BirA (BirA*) that is capable of promiscuously biotinylating proximal proteins irrespective of whether these inte...
متن کاملBioID: A Multimodal Biometric Identification System
M ost systems that control access to financial transactions, computer networks, or secured locations identify authorized persons by recognizing passwords or personal identification numbers. The weakness of these systems is that unauthorized persons can discover others' passwords and numbers quite easily and use them without detection. Biometric identification systems, which use physical feature...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2018
ISSN: 2045-2322
DOI: 10.1038/s41598-018-27500-3